Ewa Kulczykowska, Professor, Head
Tatiana Guellard, Ph.D. student
Magdalena Malinowska, Ph.D. student
Fish endocrinology: neuroendocrine regulation of fish behaviour and fish adaptation to environmental changes; arginine vasotocin and isotocin, and pineal melatonin, and their role in the control of the stress axis, and sexual behaviour of fish; new fish welfare indicators in farmed marine fish.
Principal methods: HPLC + SPE, RIA, Autoradiography, ELISA
Martins C.I.M., Galhardo L., Noble C., Damsgard B., Spedicato M.T., Zupa W., Beauchaud M., Kulczykowska E., Massabuau J.-C., Carter T., Rey Planellas S., Kristiansen T. 2012. Behavioural indicators of welfare in farmed fish. Fish Physiol. Biochem. (in press, doi: 10.1007/s10695-011-9518-8).
Almeida O., Gozdowska M., Kulczykowska E., Oliveira R.F. 2012. Brain levels of arginine-vasotocin and isotocin in dominant and subordinate males of a cichlid fish. Hormones and Behavior (in press).
Kleszczyńska A., Sokołowska E., Kulczykowska, E. (2012). Variation in brain arginine vasotocin (AVT) and isotocin (IT) levels with reproductive stage and social status in males of three-spined stickleback (Gasterosteus aculeatus). Gen. Comp. Endocrinol., 175: 290-296, (doi: 10.1016/j.ygcen.2011.11.022)
Kijewska A., Więcaszek B., Kalamarz-Kubiak H., Szulc J., Sobecka E. (2012). Skin structure studies and molecular identification of the Atlantic cod Gadus morhua L. of unique golden pigmentation from the Svalbard Bank. Journal of Applied Ichtyology, 28:60-65, (doi: 10.1111/j.1439-0426.2011.01919.x).
Kalamarz-Kubiak H., Gozdowska M., Nietrzeba M., Kulczykowska E., 2011. A novel approach to AVT and IT studies in fish brain and pituitary: In vitro perfusion technique.J. Neurosci. Methods 199 (2011) 56-61 (doi:10.1016/j.jneumeth.2011.04.035)
Sokołowska E., Fey D.P. 2011. Age and growth of the round goby Neogobius melanostomus in the Gulf of Gdańsk several years after invasion. Is the Baltic Sea a new Promised Land? J. Fish Biol. 78: 1993-2009 (doi: 10.1111/j.1095-8649.2011.02986)
Kulczykowska E., Sánchez-Vázquez F.J.: Neurohormonal regulation of feed intake and response to nutrients in fish: aspects of feeding rhythm and stress. Aquaculture Res. 41, 654-667, 2010
Sokołowski A., Bawazir A. S., Sokołowska E., Wołowicz M. 2010. Seasonal variation in the reproductive activity, physiological condition and biochemical components of the brown mussel Perna perna from the coastal waters of Yemen (Gulf of Aden). Aquat. Living Resour. 23: 177-186. (doi:10.1051/alr/2010016)
Sokołowska E., Kulczykowska E. 2009. Environmental influence on maturation and dominance relationships in the three-spined stickleback (Gasterosteus aculeatus L.): temperature competes with photoperiod for primacy. Oceanol. Hydrobiol. Stud. 38 (4): 31-48 (doi: 10.2478/v10009-009-0042-4)
Kalamarz H., Nietrzeba M., Fuentes J., Martínez-Rodríguez G., Mancera J.M., Kulczykowska E: Melatonin concentrations during larval and post-larval development of gilthead sea bream Sparus auratus L.: more than a time-keeping molecule? J. Fish Biol. 75, 142-155, 2009.
López-Olmeda J.F., Oliveira C., Kalamarz H., Kulczykowska E., Delgado M.J., Sánchez-Vázquez F.J.: Effects of water salinity on melatonin levels in plasma and peripheral tissues and on melatonin binding sites in European sea bass (Dicentrarchus labrax), Comp. Biochem. Physiol. A, 152: 486-490, 2009.
E. Kulczykowska: Arginine vasotocin and isotocin as multifunctional hormones, neurotransmitters and neuromodulators in fish. In: AVANCES en Endocrinología Comparada (eds. J.A. Munoz-Cueto, J. M. Mancera and G. Martínez-Rodriguez), Vol. IV, Servicio de Publicaciones, Universidad de Cádiz, Spain, ISBN 978-84-9828-152-1, pp.41-47, 2008.
J.F. López-Olmeda, C. Oliveira, H. Kalamarz, E. Kulczykowska, M.J.Delgado, F.J. Sánchez-Vázquez: Effects of water salinity on melatonin levels in plasma and peripheral tissues and on melatonin receptors in European sea bass (Dicentrarchus labrax). In: AVANCES en Endocrinología Comparada (eds. J.A. Munoz-Cueto, J. M. Mancera and G. Martínez-Rodriguez), Vol. IV, Servicio de Publicaciones, Universidad de Cádiz, Spain, ISBN 978-84-9828-152-1, pp.139-143, 2008.
Mancera J.M., Vargas-Chacoff L., García-López A., Kleszczyńska A., Kalamarz H., Martínez-Rodríguez G., Kulczykowska E.: High density and food-deprivation affect arginine vasotocin, isotocin and melatonin in gilthead sea bream (Sparus auratus). Comp.Biochem.Physiol. A 149, 92-97, 2008
Abbink W., Kulczykowska E., Kalamarz H., Guerreiro P., Flik G.: Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.). Gen.Comp.Endocrinol. 155, 94-100, 2008
Kulczykowska, E., 2007. Arginine Vasotocin and Isotocin: Towards Their Role in Fish Osmoregulation. In : Fish Osmoregulation, B. Baldisserotto, J. M. Mancera Romero and B.G. Kapoor (Eds.). Science Publisher, Enfield (NH), pp. 151-176.
Kulczykowska E., Kasprzak M., Kalamarz H., Kuriata M., Nietrzeba M., Jerzak L. and Kamiński P.: Melatonin and thyroxine response to pollution in white stork nestlings (Ciconia ciconia): Aspects of rhythmicity and age. Comp.Biochem.Physiol. C 146, 392-397, 2007
Kleszczyńska A., Dumont E., Cornelis R., Gozdowska M., Kulczykowska E., Sandra P.: Determination of the neuropeptides arginine vasotocin and isotocin in brains of three-spined sticklebacks (Gasterosteus aculeatus) by off-line solid phase extraction-liquid chromatography-electrospray tandem mass spectrometry. J. Chromatogr. A 1150 (1-2), 290-294 2007
Sangiao-Alvarellos S., Polakof S., Arjona F.J., Kleszczynska A., Martin del Rio M.P., Miguez J.M., Soengas J.L., Mancera J.M.: Osmoregulatory and metabolic changes in the gilthead sea bream Sparus auratus after arginine vasotocin (AVT) treatment. Gen. Comp. Endocrinol. 148, 348-358 2006.
Kleszczyńska A., Vargas-Chacoff L., Gozdowska M., Kalamarz H., Martínez-Rodríguez G., Mancera J.M., Kulczykowska E.: Arginine vasotocin, isotocin and melatonin responses following acclimation of gilthead sea bream (Sparus aurata) to different environmental salinities. Comp.Biochem.Physiol. A 145(2), 268-273, 2006.
Sokołowska E., Kulczykowska E.: Annual reproductive cycle in two free living populations of three-spined stickleback (Gasterosteus aculeatus, L.): patterns of ovarian and testicular development. Oceanologia 48(1), 103-124, 2006
Kulczykowska E., Kalamarz H., Warne J.M., Balment R.J.: Role of melatonin in fish osmoregulation: Towards a new action of the hormone. J. Exp. Zool. A, 305 (2), 145, 2006
Gozdowska M., Kleszczyńska A., Sokołowska E., Kulczykowska E.: Arginine vasotocin (AVT) and isotocin (IT) in fish brain: Diurnal and seasonal variations. Comp. Biochem. Physiol. B 143(3), 330-334, 2006
Kulczykowska E., Kalamarz H., Warne J.M., Balment R.J.: Day - night specific binding of 2-[125I]Iodomelatonin and melatonin content in gill, small intestine and kidney of three fish species. J. Comp. Physiol. B 176 (4), 277 - 285, 2006
Sokołowska E., Kalamarz H., Kulczykowska E.: Seasonal changes in brain melatonin concentration in the three-spined stickleback (Gasterosteus aculeatus): towards an endocrine calendar. Comp.Biochem.Physiol. A 139, 365-369, 2004
Kleszczyńska A., Kulczykowska E.: Determinacja płci i zachowań seksualnych u ryb: rola czynników hormonalnych i środowiskowych. Przegląd Zoologiczny 48, 29-44, 2004
Gozdowska M., Kulczykowska E.: Determination of arginine-vasotocin and isotocin in fish plasma with solid-phase extraction and fluorescence derivatization followed by high-performance liquid chromatography. J. Chromatogr. B 807, 229-233, 2004
Kulczykowska E., Sokołowska E., Takvam B., Stefansson S., Ebbesson L.: Influence of exogenous thyroxine on plasma melatonin in juvenile Atlantic salmon (Salmo salar). Comp.Biochem.Physiol. B 137(1), 43-47, 2004
Gozdowska M., Sokołowska E., Kulczykowska E.: Plasma Ca2+ concentration limits melatonin night production in two fish species. J. Fish Biol. 62(6), 1405-1413, 2003
Sokołowska E., Skóra K.E.: Reproductive cycle and the related spatial and temporal distribution of the ninespine stickleback (Pungitius pungitius L.) in Puck Bay. Oceanologia, 44(4), 475-490, 2002
Gozdowska M., Kulczykowska E.: Oznaczanie hormonów w osoczu krwi techniką HPLC. Postępy Biochemii 48(4), 317-324, 2002
Kulczykowska E.: A review of the multifunctional hormone melatonin and a new hypothesis involving osmoregulation. Rev. Fish Biol. Fish. 11(4), 321-330, 2002
Kulczykowska E.: Responses of circulating arginine vasotocin, isotocin, and melatonin to osmotic and disturbance stress in rainbow trout (Oncorhynchus mykiss). Fish Physiol. Biochem. 24, 201-206, 2001
Kulczykowska E., Warne J.M., Balment R.J.: Day-night variations in plasma melatonin and arginine vasotocin concentrations in chronically cannulated flounder (Platichthys flesus). Comp. Biochem. Physiol. A 130, 827-834, 2001
Sokołowska E., Skóra K.: Fecundity of ninespine stickleback (Pungitius pungitius L., 1758) in the Puck bay. Acta Ichthyol. Piscat. 31, 45-60, 2001
Sokołowska E., Kulczykowska E.: Biologia rozrodu ciernika Gasterosteus aculeatus (L.). Przegląd Zoologiczny 45, 197-214, 2001
Kulczykowska E.: Hormony nerwowej części przysadki mózgowej u kręgowców – rola w osmoregulacji – aspekt ewolucyjny. Przegląd Zoologiczny 44, 117-130, 2000
Kulczykowska E.: Rola hormonów przysadkowych wazotocyny argininowej i izotocyny oraz szyszynkowej melatoniny w mechanizmach adaptacyjnych u ryb kostnoszkieletowych: interakcje funkcjonalne? Crangon 2, Centrum Biologii Morza PAN, Gdynia 1999 (ISBN 83-906449-1-6)
Kulczykowska E.: Diel changes in plasma arginine vasotocin, isotocin, and melatonin in rainbow trout (Oncorhynchus mykiss). Fish Physiol. Biochem. 21, 141-146, 1999
Kulczykowska E.: Diurnal changes in arginine vasotocin and isotocin plasma levels in rainbow trout adapted to freshwater and brackish Baltic water. Ann. New York Acad. Sci. 839, 437-439, 1998
Kulczykowska E.: Effects of arginine vasotocin, isotocin and melatonin on blood pressure in the conscious Atlantic cod (Gadus morhua): hormonal interactions? Exp. Physiol. 83, 809-820, 1998
Kulczykowska E., Iuvone P.M.: Highly sensitive and specific assay of plasma melatonin using high-performance liquid chromatography with fluorescence detection preceded by solid-phase extraction. J. Chromatogr. Sci. 36, 175-178, 1998
Kulczykowska E.: Response of circulating arginine vasotocin and isotocin to rapid osmotic challenge in rainbow trout. Comp. Biochem. Physiol. A 118, 773-778, 1997
Kulczykowska E., Stolarski J.: Diurnal changes in plasma arginine vasotocin and isotocin in rainbow trout adapted to fresh water and brackish Baltic water. Gen. Comp. Endocrinol. 104, 197-202, 1996
Kulczykowska E.: Solid-phase extraction of arginine vasotocin and isotocin in fish samples and subsequent gradient reversed-phase high-performance liquid chromatographic separation. J. Chromatogr. B 673, 289-293, 1995
Kulczykowska E.: Arginine vasotocin-melatonin interactions in fish: a hypothesis. Rev. Fish Biol. Fish. 5, 96-102, 1995